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Home Antibody All anti-NIT1 antibodies

Anti-NIT1 TRUEMAB Antibody Clone OTI3A11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501084 anti-NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11) 100ul $379 In Stock
LC401717 NIT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501084 Carrier-free (BSA/glycerol-free) NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11) 100ug $450 3 Days
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WB(2)
IHC(2)
IF(2)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
Clone NameClone OTI3A11 IsotypeIgG1
Species ReactivityHuman Concentration0.87 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 35.7kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nitrilase 1 (NIT1), transcript variant 1
Alternative NameMGC57670
Database LinkNP_005591
Entrez Gene 4817 Human
FunctionPlay a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-NIT1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501084, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501084, Dilution 1:50)
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Anti-NIT1 mouse monoclonal antibody (TA501084) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1(RC211519).
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Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501084).
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HEK293T cells transfected with either pCMV6-ENTRY NIT1(RC211519)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT1 mouse monoclonal(TA501084), and then analyzed by flow cytometry.
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