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Home Antibody All anti-NIT1 antibodies

Anti-NIT1 TRUEMAB Antibody clone 4D12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501034
  • anti-NIT1 mouse monoclonal antibody, clone 4D12
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401717) , 20ug Explanation
100ul $325 In Stock
CF501034
  • Carrier-free (BSA/glycerol-free) NIT1 mouse monoclonal antibody, clone 4D12
100ug $450 3-4 weeks
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WB(1)
IHC(1)
IF(2)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
Clone Nameclone 4D12 IsotypeIgG1
Species ReactivityHuman Concentration0.66 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 35.7kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nitrilase 1 (NIT1), transcript variant 1
Alternative NameMGC57670
Database LinkNP_005591
Entrez Gene 4817 Human
FunctionPlay a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501034, Dilution 1:50)
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Anti-NIT1 mouse monoclonal antibody (TA501034) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1(RC211519).
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Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501034).
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HEK293T cells transfected with either pCMV6-ENTRY NIT1(RC211519)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT1 mouse monoclonal(TA501034), and then analyzed by flow cytometry.
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