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Anti-NIT1 TRUEMAB Antibody Clone OTI4D12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501034 anti-NIT1 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12) 100ul 325
In Stock
LC401717 NIT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501034 Carrier-free (BSA/glycerol-free) NIT1 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
Clone NameClone OTI4D12 IsotypeIgG1
Species ReactivityHuman Concentration0.66 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 35.7kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nitrilase 1 (NIT1), transcript variant 1
Alternative NameMGC57670
Database LinkNP_005591
Entrez Gene 4817 Human
FunctionPlay a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (RC211519, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501034, Dilution 1:50)
Anti-NIT1 mouse monoclonal antibody (TA501034) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1(RC211519).
Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (TA501034).
HEK293T cells transfected with either pCMV6-ENTRY NIT1(RC211519)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT1 mouse monoclonal(TA501034), and then analyzed by flow cytometry.


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