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Anti-NGF Antibody EP1320Y
|A synthetic peptide corresponding to residues near the C-terminus of human NGF-? was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens nerve growth factor (beta polypeptide) (NGF)|
|Beta-NGF; HSAN5; NGFB|
|Nerve growth factor (NGF) stimulates division and differentiation of sympathetic and embryonic sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurons (1). NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. While NGF is found in almost all vertebrates, most research has focused on murine NGF, as the mouse male submaxillary gland contains higher levels of this polypeptide than other tissues (2). |
MAPK signaling pathway
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Western blot - NGF antibody [EP1320Y]; Anti-NGF antibody [EP1320Y] at 1/500 dilution + fetal thyroid tissue at 10 µg.Secondary.Goat anti rabbit IgG HRP antibody at 1/2000 dilution.Predicted band size : 27 kDa.Observed band size : 27 kDa.
Immunohistochemistry (Paraffin-embedded sections) - NGF antibody [EP1320Y]; Ab52918 at 1/50-1/100 dilution staining human brain; paraffin embedded.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NGF antibody [EP1320Y]; ab52918 stainingNGF inMouse tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections wereformaldehyde fixed, subjected to heat mediated antigen retrieval and blocked for10 minutes at 25C.The primary antibody was diluted1/50and incubated with the sample for 1 hour at 25°C. An HRP polymeranti-rabbit IgG system was used undiluted, as the secondary antibody.