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Anti-NFKBIB PHOSPHO Antibody
Also for NFKBIB (NM_002503)
|The antiserum was produced against synthesized phosphopeptide derived from human I?B-ß around the phosphorylation site of serine 23.|
|Human: Ser23, Mouse: Ser23, Rat: Ser23
|WB, IHC, IF
||WB: 1:500~3000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|I.B-. (Phospho-Ser23) antibody detects endogenous levels of I.B-. only when phosphorylated at serine 23.
|Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (NFKBIB), transcript variant 1|
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Western blot analysis of extracts from Hela cells treated with TNF-? (20ng/ml, 30mins), using I?B-ß (Phospho-Ser23) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using I?B-ß (Phospho-Ser23) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using I?B-ß (Phospho-Ser23) antibody.The picture on the right is treated with the synthesized peptide.