Home Antibody All anti-NFKBIA antibodies
Also for NFKBIA (NM_020529)
|Recombinant fragment corresponding to a region within amino acids 1 and 317 of IKB alpha (Uniprot ID#P25963)|
|Human (Predicted: Rhesus Monkey)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Rhesus Monkey (100%)
|Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)|
|IKBA; MAD-3; NFKBI|
Entrez Gene 4792 Human
Entrez Gene 695335 Monkey
|NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]|
|Druggable Genome Chemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathwayMore Pathways >> |
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IKB alpha antibody detects IKB alpha protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. B. 30 ug C2C12 whole cell lysate/extrac. 10 % SDS-PAGE. IKB alpha antibody (TA308928) dilution: 1:5000
Sample (30 ug of whole cell lysate). A: Hep G2 . 10% SDS PAGE. TA308928 diluted at 1:1000.
IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody (TA308928) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(TA308928) antibody at 1:200 dilution.