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Home Antibody All anti-NFKBIA antibodies

Anti-NFKBIA Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308928
  • Rabbit polyclonal antibody to IkappaB-alpha (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412432) , 20ug Explanation
100ul 325 3-7 Days
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WB(2)
IHC(1)
IF(1)
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Also for NFKBIA (NM_020529)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 1 and 317 of IKB alpha (Uniprot ID#P25963)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Rhesus Monkey) Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
Predicted MW Explanation 36kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

Target NameHomo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)
Alternative NameIKBA; MAD-3; NFKBI
Database LinkNP_065390
Entrez Gene 4792 Human
Entrez Gene 695335 Monkey
FunctionNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Related PathwayDruggable Genome Chemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
IKB alpha antibody detects IKB alpha protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. B. 30 ug C2C12 whole cell lysate/extrac. 10 % SDS-PAGE. IKB alpha antibody (TA308928) dilution: 1:5000
WB Image
Sample (30 ug of whole cell lysate). A: Hep G2 . 10% SDS PAGE. TA308928 diluted at 1:1000.
IHC Image
IKB alpha antibody detects NFKBIA protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. IKB alpha antibody (TA308928) dilution: 1:500.
IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(TA308928) antibody at 1:200 dilution.

 

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