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Anti-NEK7 Antibody EPR4900
Also for NEK7 (NM_133494)
|A synthetic peptide corresponding to residues in human NEK7 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens NIMA-related kinase 7 (NEK7)|
|NIMA-related kinase 7; OTTHUMP00000033680; NIMA (never in mitosis gene a)-related kinase 7|
|NIMA-related kinases such as NEK7 share high amino acid sequence identity with a serine/threonine kinase identified in Aspergillus nidulans, which controls initiation of mitosis (1). NEK7 acts together with NEK6 and NEK9 in a mitotic kinase cascade. (2). It can also localize to the centrosome in both interphase and mitotic U2OS and HeLa cells. Thus, in common with other NIMA-related kinases implicated in mitotic regulation, NEK7 is localized to the MTOC at the time of mitotic entry (3).|
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Western blot - Anti-NEK7 antibody [EPR4900]; All lanes : Anti-NEK7 antibody [EPR4900] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : A549 cell lysate.Lane 3 : C6 cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : PC12 cell lysate.Lane 6 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat-anti-rabbit HRP at 1/2000 dilution.developed using the ECL technique.Predicted band size : 35 kDa.Observed band size : 32 kDa .