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Anti-NEFH Antibody EP673Y
Also for NEFH (NM_021076)
|A synthetic peptide corresponding to residues in human NF-H was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:2,000; IHC: 1:500; ICC: 1:100-250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens neurofilament, heavy polypeptide (NEFH)|
|Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]. |
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Western blot - 200 kD Neurofilament Heavy antibody [EP673Y]; Anti-200 kD Neurofilament Heavy antibody [EP673Y] at 1/2000 dilution + Rat brain lysate.Predicted band size : 112 kDa.Observed band size : 200 kDa .Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - 200 kD Neurofilament Heavy antibody [EP673Y]; Immunohistochemical analysis of paraffin embedded human brain glyoma using ab40796.
Immunofluorescence - 200 kD Neurofilament Heavy antibody [EP673Y]; Immunofluorescent staining of PC-12 cells using ab40796.