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Home Antibody All anti-NEDD8 antibodies

Anti-NEDD8 Antibody Y297

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303612
  • Rabbit monoclonal antibody against NEDD8 (N-term) (Y297 )
100ul 325 3-7 Days
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WB(1)
IP(1)
IHC(1)
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Also for NEDD8 (NM_006156)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.
Clone NameY297 IsotypeTissue culture supernatant
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; IP: 1:50
BufferDoes not react with Mouse. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens neural precursor cell expressed, developmentally down-regulated 8 (NEDD8)
Alternative NameNEDD-8
Database LinkNP_006147
Entrez Gene 4738 Human
Entrez Gene 18002 Mouse
Entrez Gene 25490 Rat
Function
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - NEDD8 antibody [Y297]; All lanes : Anti-NEDD8 [Y297] antibody at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 9 kDa.Observed band size : 9 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NEDD8 antibody [Y297]; TA303612, at 1/100 dilution, staining NEDD8 in human lung carcinoma tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded section.
IP Image
Immunoprecipitation - NEDD8 antibody [Y297]; Western blot analysis of immunoprecipitation pellet from HeLa cell lysate using TA303612 at 1/500 dilution. Band at 90 kDa shows Neddylated cullins, 50 kDa the IgG fraaction and 9 kDa Free NEDD8.

 

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