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Home Antibody All anti-NECAB1 antibodies

Anti-NECAB1 TRUEMAB Antibody Clone OTI2H5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502630 NECAB1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5) 100ul $325 In Stock
LC411679 NECAB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502630 Carrier-free (BSA/glycerol-free) NECAB1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5) 100ug $450 3-4 weeks
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WB(2)
FC(2)
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Also for NECAB1 (NM_022351)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NECAB1 (NP_071746) produced in HEK293T cell.
Clone NameClone OTI2H5 IsotypeIgG1
Species ReactivityHuman Concentration0.61 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:2000, FLOW 1:100
Predicted MW Explanation 40.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens N-terminal EF-hand calcium binding protein 1 (NECAB1)
Alternative NameEFCBP1; STIP-1
Database LinkNP_071746
Entrez Gene 64168 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NECAB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502630), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-NECAB1 antibody(TA502630),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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