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Anti-NCK1 Antibody Y531
Also for NCK1 (NM_006153)
|A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:2000 - 1:10000; IHC-P: Use at an assay dependent concentration; ICC: 1:250 - 1:500; FC: 1:1000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - Nck antibody [Y531]; Anti-Nck antibody [Y531] at 1/10000 dilution + Hela cell lysate.Observed band size : 47 kDa .Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531]; TA303611, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
Flow Cytometry - Anti-Nck antibody [Y531]; Overlay histogram showing Jurkat cells stained with TA303611 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.