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Home Antibody All anti-NAPEPLD antibodies

Anti-NAPEPLD TRUEMAB Antibody Clone OTI5F7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA503860 NAPEPLD (NAPE-PLD) mouse monoclonal antibody, clone OTI5F7 (formerly 5F7) 100ul $379 In Stock
LC404703 NAPEPLD HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503860 Carrier-free (BSA/glycerol-free) NAPEPLD mouse monoclonal antibody, clone OTI5F7 (formerly 5F7) 100ug $450 3-4 weeks
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WB(2)
IHC(6)
IF(1)
FC(1)
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Also for NAPEPLD (NM_198990)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NAPEPLD(NP_945341) produced in HEK293T cell.
Clone NameClone OTI5F7 IsotypeIgG2a
Species ReactivityHuman Concentration0.71 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 45.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2
Alternative NameFMP30; NAPE-PLD
Database LinkNP_945341
Entrez Gene 222236 Human
FunctionNAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPEPLD (RC209877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPEPLD.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NAPEPLD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503860)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503860)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503860)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503860)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503860)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503860)
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Anti-NAPEPLD mouse monoclonal antibody (TA503860) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NAPEPLD(RC209877).
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HEK293T cells transfected with either RC209877 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NAPEPLD antibody(TA503860), and then analyzed by flow cytometry.
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