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Also for NAPA (NM_003827)
|Recombinant fragment corresponding to a region within amino acids 78 and 295 of alpha SNAP (Uniprot ID#P54920)|
|Human, Mouse (Predicted: Rhesus Monkey, Chimpanzee)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Rhesus Monkey (100%), Chimpanzee (100%)
|Homo sapiens N-ethylmaleimide-sensitive factor attachment protein, alpha (NAPA), transcript variant 1|
Entrez Gene 8775 Human
Entrez Gene 108124 Mouse
Entrez Gene 717355 Monkey
|The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different. [provided by RefSeq]|
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Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308674 diluted at 1:10000.
Sample(30 ug whole cell lysate). A:Hep G2 . 10% SDS PAGE. TA308674 diluted at 1:1000
alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] (TA308674) dilution: 1:500.
Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP(TA308674) antibody at 1:200 dilution.