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Home Antibody All anti-MTOR antibodies

Anti-MTOR Antibody EPR390(N)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310699
  • Rabbit Monoclonal antibody against mTOR/FRAP
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417627) , 20ug
100ul 325 3-7 Days
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WB(1)
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Also for MTOR (NM_004958)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human mTOR/FRAP was used as an immunogen.
Clone NameEPR390(N) IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:10000
BufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Reference Data

Target NameHomo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)
Alternative NameFRAP; FRAP1; FRAP2; RAFT1; RAPT1
Database LinkNP_004949
FunctionThe mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and through the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway (1).
Related Pathway
Senescence and Autophagy

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WB Image
Western blot - Anti-mTOR antibody [EPR390(N)]; All lanes : Anti-mTOR antibody [EPR390(N)] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : Raji cell lysate.Lane 4 : HepG2 cell lysate.Lane 5 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution.Predicted band size : 289 kDa.Observed band size : 289 kDa.

 

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