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Home Antibody All anti-MMP12 antibodies

Anti-MMP12 Antibody EP1261Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300810
  • Rabbit Monoclonal Antibody against MMP12 (Clone EP1261Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400868) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for MMP12 (NM_002426)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human MMP-12 was used as an immunogen.
Clone NameEP1261Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:30; FC: 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens matrix metallopeptidase 12 (macrophage elastase) (MMP12)
Alternative NameHME; ME; MME; MMP-12
Database LinkNP_002417
FunctionHuman macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MMP12 antibody [EP1261Y] - Carboxyterminal end; .Predicted band size : 54 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MMP12 antibody [EP1261Y]; Ab52897 (1:100) staining human MMP12 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - MMP12 antibody [EP1261Y]; Immunofluorescent staining of HeLa cells using TA300810 (1:100).
FC Image
Flow Cytometry - MMP12 antibody [EP1261Y] - Carboxyterminal end; Overlay histogram showing A549 cells stained with TA300810 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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