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Home Antibody All anti-MME antibodies

Anti-MME Antibody EPR2997

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307130
  • Rabbit monoclonal antibody against CD10 (clone EPR2997 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416064) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for MME (NM_007287)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human CD10 was used as an immogen.
Clone NameEPR2997 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:5000 - 1:10000; FC: 1:100 - 1:150
Predicted MW Explanation 86 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens membrane metallo-endopeptidase (MME), transcript variant 1bis
Alternative NameCALLA; CD10; NEP; SFE
Database LinkNP_009218
Entrez Gene 4311 Human
Entrez Gene 17380 Mouse
Entrez Gene 24590 Rat
FunctionCD10 is zinc-dependant metalloprotease enzyme that plays a role in the destruction of opiod peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (1). CD10 is a cell-surface glycoprotein expressed on most acute lymphoblastic leukemias and certain other immature lymphoid malignancies and on normal lymphoid progenitors. The latter are either uncommitted to B- or T-cell lineage or committed to only the earliest stages of B- or T-lymphocyte maturation. CD10 is not restricted to leukemic cells, however, and is found on a variety of normal tissues. CD10 is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium (2). The proteolysis of beta-amyloid (Abeta) requires CD10, an enzyme that has been shown as reduced in Alzheimer's disease (AD). The defect of CD10 appears to correlate with Abeta deposition but not with degeneration and dementia (3).
Related PathwayTransmembraneProteaseDruggable Genome Renin-angiotensin systemHematopoietic cell lineageAlzheimer's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CD10 antibody [EPR2997]; All lanes : Anti-CD10 antibody [EPR2997] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : LnCap cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 86 kDa.Observed band size : 100 kDa .
FC Image
Flow Cytometry-CD10 antibody [EPR2997](TA307130); Overlay histogram showing Jurkat cells stained with TA307130 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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