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Anti-MKI67 Antibody EPR3611
Also for MKI67 (NM_002417)
|A synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|IHC, IF, FC
||IHC-P: Use at an assay dependent dilution; ICC/IF: 1:500 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ,IP or WB.
|Homo sapiens marker of proliferation Ki-67 (MKI67), transcript variant 1|
|Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is consider an important marker in human tumor diagnostics and prognostics (2). |
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ki67 antibody [EPR3611]; ab92353 detecting Ki67 in paraffin-embedded human tonsils (1) and paraffin-embedded mouse intestine (2) by immunohistochemistry; Note nuclear staining of proliferating cells in sample 1.
Immunocytochemistry/ Immunofluorescence - Ki67 antibody [EPR3611]; ab92353 at 1/500 dilution staining Ki67 in HeLa cells by immunofluorescence.
Flow Cytometry - Ki67 antibody [EPR3611]; ab92353 at 1/30 dilution detecting Ki67 in permeabilized HeLa cells by flow cytometry. Detection utilised ab92353 (red) or a rabbit IgG (negative)(green).