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Anti-MET PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human c-Met around the phosphorylation site of tyrosine 1003 (V-D-YP-R-A).|
|Human: Tyr1003, Mouse: Tyr1001, Rat: Tyr1004
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|c-Met (Phospho-Tyr1003) antibody detects endogenous levels of c-Met only when phosphorylated at tyrosine 1003.
|Homo sapiens met proto-oncogene (MET), transcript variant 2|
|AUTS9; c-Met; HGFR; RCCP2|
Cytokine-cytokine receptor interaction
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Western blot analysis of extracts from HepG2 cells, using c-Met (Phospho-Tyr1003) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using c-Met (Phospho-Tyr1003) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using c-Met (Phospho-Tyr1003) antibody.The picture on the right is treated with the synthesized peptide.