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Anti-MELK Antibody EPR3981
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Also for MELK (NM_014791)
|A synthetic peptide corresponding to residues in human MELK was used as an immunogen.|
||WB: 1:1,000 - 10,000,
|Store at -20 C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.|
|Homo sapiens maternal embryonic leucine zipper kinase (MELK), transcript variant 1|
|Maternal embryonic leucine zipper kinase (MELK), a member of the CAMK Serine/Threonine protein kinase family, functions to regulate the cell cycle (1). It serves as a marker for self-renewing multipotent neural progenitors (MNP) (1). MELK interacts in a phosphorylation-dependent manner with the transcription and splicing factor NIPP1, and this interaction can inhibit spliceosome assembly during mitosis (2). An additional substrate of MELK is the zinc finger-like protein ZPR9, which binds tightly to MELK and causes its nuclear accumulation (3). It is also expressed in tissues such as the placenta, kidney, and thymus (4).|
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Western blot analysis on (A) K562, (B) 293T, and (C) Jurkat cell lysates using anti-MELK RabMAb .