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Anti-ME2 Antibody EPR7218
Also for ME2 (NM_001168335)
|A synthetic peptide corresponding to residues in human ME2 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
|Does not react with Mouse. Is unsuitable for Flow Cyt or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens malic enzyme 2, NAD(+)-dependent, mitochondrial (ME2), transcript variant 2|
Entrez Gene 4200 Human
|ME2 is a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different protein. Certain mutations of ME2 have been shown to increase the risk for idiopathic generalized epilepsy (1). |
| Pyruvate metabolism|
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Western blot - Anti-ME2 antibody [EPR7218]; All lanes : Anti-ME2 antibody [EPR7218] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : K562 cell lysate.Lane 5 : MOLT4 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 65 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EPR7218]; TA310683, at 1/50 dilution, staining ME2 in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-ME2 antibody [EPR7218]; TA310683, at 1/100 dilution, staining ME2 in HeLa cells by Immunofluorescence.