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Anti-MCM2 PHOSPHO Antibody EPR4119
Also for MCM2 (NM_004526)
|A phospho specific peptide corresponding to residues surrounding serine 27 of human MCM2 was used as an immunogen. This antibody only detects MCM2 phosphorylated at serine 27.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens minichromosome maintenance complex component 2 (MCM2), transcript variant 1|
|BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN|
|Minichromosome maintenance protein 2 (MCM2) is a member of the MCM family that regulates mammalian DNA replication (1). This family is composed of six related subunits, called the hexameric MCM2-7 complex, that are conserved in all eukaryotes. It functions as a replicative helicase, the molecular motor that both unwinds duplex DNA and powers fork progression during DNA replication (2). During cell division, MCM2 is required for the entry into S phase (3). It acts as a factor to license DNA for one and only one round of replication per cell cycle (3). MCM2 physically interacts with and is phosphorylated by Cdc7/Dbf4, thus regulating the initiation of DNA replication (4). Ser27, Ser41, and Ser139 are suggested to be Cdc7/Dbf4 phosphorylation sites in MCM2 (5).|
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Western blot - MCM2 (phospho S27) antibody [EPR4119]; All lanes : Anti-MCM2 (phospho S27) antibody [EPR4119] at 1/10000 dilution.Lane 1 : HeLa cell lysates untreated with alkaline phosphatase .Lane 2 : HeLa cell lysates treated with alkaline phosphatase .Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody [EPR4119]; ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM2 (phospho S27) antibody [EPR4119]; ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - MCM2 (phospho S27) antibody [EPR4119]; ab109459 at 1/100 dilution staining MCM2 (phospho S27) in HeLa cells by Immunofluorescence.