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Home Antibody All anti-MAPKAPK2 antibodies

Anti-MAPKAPK2 Antibody E341

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SKU Description Amount Price Availability*  
TA303762
  • Rabbit monoclonal antibody against MAPKAP Kinase-2 (E341)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417772) , 20ug
100ul 325 In Stock
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WB(2)
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Also for MAPKAPK2 (NM_004759)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2 was used as immunogen.
Clone NameE341 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:500-1,000; IP: 1:200
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 1
Alternative NameMAPKAP-K2; MK-2; MK2
Database LinkNP_004750
FunctionThis gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway
MAPK signaling pathway

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WB Image
Western blot - MAPKAP Kinase 2 antibody [E341]; Anti-MAPKAP Kinase 2 antibody [E341] at 1/1000 dilution + Hela cell lysate.Predicted band size : 46 kDa.Observed band size : 46 kDa.
WB Image
Western blot - Anti-MAPKAP Kinase 2 antibody [E341]; Lanes 1 - 2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] at 1/500 dilution.Lanes 3 - 4 : Anti-MAPKAP Kinase 2 antibody [E341] at 1/500 dilution.Lane 1 : UV treated HeLa cells.Lane 2 : UV treated HeLa cells + p38 inhibitor.Lane 3 : UV treated HeLa cells.Lane 4 : UV treated HeLa cells + p38 inhibitor.Secondary.IRDye? 680 LICOR goat anti-rabbit.Performed under reducing conditions.Predicted band size : 46 kDa.Observed band size : 48 kDa .Additional bands at : 55 kDa (possible cross reactivity).Exposure time : 1 minute

 

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