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Home Antibody All anti-MAPK1 antibodies

Anti-MAPK1 PHOSPHO Antibody EPR5463

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310674
  • Rabbit Monoclonal antibody against MARK1, pT215 (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
Assay(1)
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Also for MAPK1 (NM_002745)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 215 of human MARK1 was used as an immunogen. This antibody only detects MARK1 phosphorylated at Threonine 215.
Clone NameEPR5463 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY Suggested DilutionsWB: 1:10000 - 1:50000
Predicted MW Explanation 89 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1
Alternative NameERK; ERK-2; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
Database LinkNP_002736
Entrez Gene 5594 Human
Entrez Gene 26413 Mouse
Entrez Gene 116590 Rat
FunctionMARK belongs to the AMPK (adenosine-monophosphate activated protein kinase) branch of the CAMK (calcium/calmodulin-dependent protein kinase) group of kinases (1). MARK1 has an important neuronal function, like other MARK proteins (2). It phosphorylates Tau and related microtubule-associated proteins (MAPs) at specific sites in the microtubule binding repeats. Phosphorylation of Tau by MARK1 results in the detachment from microtubules and affects microtubule dynamics and microtubule-based intracellular transport (3). MARK1 is activated by phosphorylation on Threonine 215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39 (4). Moreover, hyperphosphorylation by MARK1 is implicated in the initial step for the abnormal aggregation of Tau in Alzheimer’s disease (3).
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-MARK1 (phospho T215) antibody [EPR5463]; All lanes : Anti-MARK1 (phospho T215) antibody [EPR5463] at 1/10000 dilution.Lane 1 : SH-SY5Y cell lysate, untreated.Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A .Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 89 kDa.
Assay Image
Other-Anti-MARK1 (phospho T215) antibody [EPR5463](TA310674); Equilibrium disassociation constant (KD)..

 

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