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Home Antibody All anti-MAP3K3 antibodies

Anti-MAP3K3 Antibody ep600y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303595
  • Rabbit monoclonal antibody against MEKK3 (C-term)(EP600Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419352) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for MAP3K3 (NM_002401)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human MEKK3 was used as immunogen.
Clone Nameep600y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution; WB: 1:1000; FC: 1:20; IP: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2
Alternative NameMAPKKK3; MEKK3
Database LinkNP_002392
FunctionThis gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway

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WB Image
Western blot - MEKK3 antibody [ep600y]; Anti-MEKK3 antibody [ep600y] at 1/1000 dilution + HeLa cell lysate at 10 µg.Predicted band size : 71 kDa.Observed band size : 70 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MEKK3 antibody [ep600y]; Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using TA303595 diluted 1:100
IF Image
Immunofluorescence - MEKK3 antibody [ep600y]; Immunofluorescent staining of HeLa cells using TA303595 diluted 1:100

 

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