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Anti-MAP3K2 Antibody EP626Y
Also for MAP3K2 (NM_006609)
|A synthetic peptide corresponding to residues near the N-term of human MEKK-2 was used as immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IP, FC
||IHC-Fr: Use at an assay dependent dilution; WB: 1:10000 - 1:50000; ICC: 1:250 - 1:500; IHC-P: Use at an assay dependent dilution; IP: Use at an assay dependent concentration; FC: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens mitogen-activated protein kinase kinase kinase 2 (MAP3K2)|
|MEKK2 is a Ser/Thr protein kinase belonging to the MEKK/STE11 subgroup of the MAP/ERK kinase kinase family (1). Closely related to MEKK3, MEKK2 is expressed in a multitude of tissues. MEKK2 directly phosphorylates and activates Ikappa B kinases and has been found to bind and activate protein kinase C-related kinase 2 (2). MEKK2 has also been known to activate JNK1/2, ERK1/2, p38, and ERK5 MAPK in vitro (3). MEKK2 interacting protein (Mip1) regulates MEKK2 expression (4).|
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - MEKK2 antibody [EP626Y]; Anti-MEKK2 antibody [EP626Y] at 1/50000 dilution + Jurkat cell lysate.Predicted band size : 70 kDa.Observed band size : 70 kDa.
Immunohistochemistry (Paraffin-embedded sections) - MEKK2 antibody [EP626Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA300584 at 1/50.
Immunoprecipitation - Anti-MEKK2 antibody [EP626Y]; MEKK2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 10Âµg of Rabbit monoclonal [EP626Y] to MEKK2 and 50Âµl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40Âµl SDS loading buffer and incubated for 10min at 70oC; 10Âµl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300584.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 75kDa: MEKK2.
Flow Cytometry - MEKK2 antibody [EP626Y]; Overlay histogram showing HepG2 cells stained with TA300584 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.