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Anti-MAP2K7 PHOSPHO Antibody
Also for MAP2K7 (NM_145185)
|The antiserum was produced against synthesized phosphopeptide derived from human MAP2K7 around the phosphorylation site of threonine 275 (A-K-TP-R-S).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|MAP2K7 (Phospho-Thr275) antibody detects endogenous levels of MAP2K7 only when phosphorylated at threonine 275.
|Homo sapiens mitogen-activated protein kinase kinase 7 (MAP2K7), transcript variant 3|
|JNKK2; MAPKK7; MEK; MEK 7; MKK7; PRKMK7; SAPKK-4; SAPKK4|
EGFR1 Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MAP2K7 (Phospho-Thr275) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody. The lane on the left is treated with synthesized peptide.