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Anti-MAP2K4 TRUEMAB Antibody Clone OTI2D5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for MAP2K4 (NM_003010)
|Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector|
|WB, IHC, IF, IP
||WB 1:2000, IHC 1:150, IF 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500430)
Anti-MAP2K4 mouse monoclonal antibody (TA500430) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4(RC206051).
Immunoprecipitation(IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.