Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas Blood, Mar 2013; 121: 2563 - 2566.
[anti-HA]
Regulation of the PI3-K/Akt Survival Pathway in the Rat Endometrium Biol Reprod, Mar 2013; 88: 79.
[Akt3]
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt159.
[LA]
Ruxolitinib as potential targeted therapy for patients with JAK2 rearrangements Haematologica, Mar 2013; 98: 404 - 408.
[JAK2]
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S257of human MAP2K4.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
IgG
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1: 10~50; IHC: 1:50~100; ELISA: 1: 1,000
Background
MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Related Pathway
Apoptosis
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot analysis of MAP2K4 (arrow) using rabbit polyclonal MAP2K4 Antibody (S257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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