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Home Antibody All anti-MAP2K1 antibodies

Anti-MAP2K1 PHOSPHO Antibody EPR3338

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307325
  • Rabbit monoclonal antibody against MEK-1 Phospho (pS298) (clone EPR3338 ) (Phospho-Specific)
100ul 325 In Stock
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WB(1)
IHC(4)
IF(14)
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding serine 298 of human MEK1 were used as an immunogen. This antibody detects MEK1 phosphorylated at threonine 298.
Clone NameEPR3338 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; IHC-Fr: 1:100 - 1:250
Predicted MW Explanation 43 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
Entrez Gene 5604 Human
Entrez Gene 26395 Mouse
Entrez Gene 170851 Rat
FunctionMEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). p21-activated kinases (Paks) regulate MEK-1 activity, by phosphorylating proteins at Ser(298). Mek1 S298 phosphorylation is necessary for efficient activation of MEK1 and subsequent MAPK activation (5).
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosisVascular smooth muscle contractionDorso-ventral axis formationMore Pathways >>

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WB Image
Western blot - MEK1 (phospho S298) antibody [EPR3338]; All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] at 1/1000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : HeLa cell lysates treated with LP.Lysates/proteins at 10 ug per lane.Secondary.HRP/AP polymerized antibody.Predicted band size : 43 kDa.Observed band size : 45 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEK1 (phospho S298) antibody [EPR3338]; TA307325, at a 1/100 dilution, staining MEK1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemical analysis.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 showing positive staining in Thyroid gland carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 showing positive staining in Ovarian carcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-Williardine , by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-Williardine, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ((S)-Williardine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in the NIH3T3 cell line fromMouse fibroblastsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor?488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - MEK1 (phospho S298) antibody [EPR3338]; TA307325, at a 1/100 dilution, staining MEK1 in HeLa cells by Immunofluorescent analysis.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX disodium salt , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX disodium salt, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (NBQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX disodium salt , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX disodium salt, as described in literature.The cells were incubated at 37°C for 3h in media containing different concentrations of (DNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-MEK1 (phospho S298) antibody [EPR3338](TA307325); TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Chlorowillardiine , by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Chlorowillardiine, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of ((S)-5-Chlorowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-MEK1 (phospho S298) antibody [EPR3338](TA307325); TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Nitrowillardiine , by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Nitrowillardiine, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of ((S)-5-Nitrowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence-Anti-MEK1 (phospho S298) antibody [EPR3338](TA307325); TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with (R,S)-AMPA , by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of(R,S)-AMPA, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of ((R,S)-AMPA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-AMPA , by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-AMPA, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of ((S)-AMPA) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with GYKI 52466 , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of GYKI 52466, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of (GYKI 52466) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S298) antibody [EPR3338]; TA307325 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX , by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX, as described in literature.The cells were incubated at 37°C for 1h in media containing different concentrations of (DNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA307325 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody.

 

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