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Home Antibody All anti-MAP2K1 antibodies

Anti-MAP2K1 PHOSPHO Antibody EP2364Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301190
  • Rabbit Monoclonal Antibody against MAP2K1 (Clone EP2364Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding threonine 286 of human MEK1 were used as an immunogen. This antibody detects MEK1 phosphorylated at threonine 286.
Clone NameEP2364Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:2000; IP: 1:50
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
FunctionMEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). report the phosphorylation and inactivation of MKK1 by phosphorylation on threonine 286 and threonine 292. MKK1 contains a consensus phosphorylation site for p34cdc2, a serine/threonine protein kinase that regulates the cell division cycle, at Thr-286 and a related site at Thr-292 (5).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
Senescence and Autophagy
Toll-like receptor signaling pathway

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WB Image
Western blot - MEK1 (phospho T286) antibody [EP2364Y]; All lanes : Anti-MEK1 (phospho T286) antibody [EP2364Y] at 1/2000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with nocodazole.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.Observed band size : 43 kDa.

 

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