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Home Antibody All anti-MAP2K1 antibodies

Anti-MAP2K1 PHOSPHO Antibody EP2364Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301190
  • Rabbit Monoclonal Antibody against MAP2K1 (Clone EP2364Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding threonine 286 of human MEK1 were used as an immunogen. This antibody detects MEK1 phosphorylated at threonine 286.
Clone NameEP2364Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:2000; IP: 1:50
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
FunctionMEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). report the phosphorylation and inactivation of MKK1 by phosphorylation on threonine 286 and threonine 292. MKK1 contains a consensus phosphorylation site for p34cdc2, a serine/threonine protein kinase that regulates the cell division cycle, at Thr-286 and a related site at Thr-292 (5).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
Senescence and Autophagy
Toll-like receptor signaling pathway

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WB Image
Western blot - MEK1 (phospho T286) antibody [EP2364Y]; All lanes : Anti-MEK1 (phospho T286) antibody [EP2364Y] at 1/2000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with nocodazole.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.Observed band size : 43 kDa.

 

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