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Home Antibody All anti-MAP1LC3A antibodies

Anti-MAP1LC3A Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324624
  • Rabbit polyclonal LC3 Antibody (APG8A) (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403169) , 20ug
400ul 325 3-7 Days
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WB(2)
IHC(1)
IF(1)
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Also for MAP1LC3A (NM_032514)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000; IHC: 1:50-100; IF: 1:200
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1
Alternative NameATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Database LinkNP_115903
Function
Related Pathway
Senescence and Autophagy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
WB Image
APG8a (MAP1LC3A) Antibody (M1) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IF Image
Fluorescent image of U251 cells stained with TA324624 LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine (50 .M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324624 LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 .g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>

 

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