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Anti-LRP1 Antibody EPR3724
Also for LRP1 (NM_002332)
|A synthetic peptide corresponding to residues in human LRP1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||IHC-P: 1:100 - 1:250; WB: 1:20000 - 1:50000; IP: 1:50; FC: 1:1000; ICC/IF: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens low density lipoprotein receptor-related protein 1 (LRP1)|
|A2MR; APOER; APR; CD91; IGFBP3R; LRP; LRP1A; TGFBR5|
|The LDL receptor-related protein (LRP1) is a large endocytic receptor that is widely expressed in several tissues. LRP1 is a member of the LDL receptor family that plays diverse roles in various biological processes including lipoprotein metabolism, degradation of proteases, activation of lysosomal enzymes, and cellular entry of bacterial toxins and viruses. LRP1 can recognize more than 30 distinct ligands, bind a large number of cytoplasmic adaptor proteins via determinants located on its cytoplasmic domain in a phosphorylation-specific manner, and can associate with and modulate the activity of other transmembrane receptors such as integrins and receptor tyrosine kinases (1). This multi-talented receptor has been implicated in regulation of platelet derived growth factor receptor activity, integrin maturation and recycling, and focal adhesion disassembly (2). |
Wnt Signaling Pathway
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Western blot - Low Density LRP antibody [EPR3724]; All lanes : Anti-LRP1 [EPR3724] antibody at 1/20000 dilution.Lane 1 : Human PMBC lysate.Lane 2 : A549 lysate.Lane 3 : Mouse brain lysate.Lane 4 : Mouse heart lysate.Lane 5 : Mouse kidney lysate.Lane 6 : Mouse spleen lysate.Lane 7 : Rat brain lysate.Lane 8 : Rat heart lysate.Lane 9 : Rat kidney lysate.Lane 10 : Rat spleen lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Low Density LRP antibody [EPR3724]; ab92544 at 1/100 dilution staining Low Density LRP in formalin-fixed, paraffin-embedded Human liver tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724]; ab92544 showing positive staining in Normal brain tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724]; ab92544 showing positive staining in Normal lung tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724]; ab92544 showing positive staining in Normal placenta tissue.
Immunocytochemistry/ Immunofluorescence - Low Density LRP antibody [EPR3724]; ab92544 at 1/100 dilution staining Low Density LRP in HEPG2 cells by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [EPR3724]; ICC/IF image of LRP1 staining on rat mixed glia culture using ab92544 (1:200). The cells were fixed using paraformaldehyde. The cells were then permeabilised using 0.1% TritonX in 0.1% PBS. Non-specific protein was blocked using 10% donkey serum at 24°C for 1 hour. Ab92544 was diluted(1:200) using 0.1% TritonX with 0.1% PBS and 10% donkey serum and the cells were incubated for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to Rabbit IgG conjugated to Alexa Fluor 488. DAPI was used to stain the nucleus.
Flow Cytometry - Anti-LRP1 antibody [EPR3724]; Overlay histogram showing Jurkat cells stained with ab92544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.