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Anti-LPIN1 Antibody EPR3725
Also for LPIN1 (NM_145693)
|A synthetic peptide corresponding to residues in human Lipin-1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:20; IHC-P: 1:50 - 1:100; ICC: 1:100 - 1:250; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens lipin 1 (LPIN1), transcript variant 1|
|Lipin-1 is a Mg(2)+-dependent phosphatidate phosphatase (PAP) enzyme which catalyzes the conversion of phosphatidate to diacylglycerol, a key reaction in glycerolipid biosynthesis (1). It plays critical roles in controlling energy metabolism, has establishing the adipogenic gene expression program during early stages of differentiation, and in triglyceride accumulation in mature adipocytes (2, 3). Lipin-1 also functions as a transcriptional coactivator in conjunction with members of the peroxisome proliferator-activated receptor (PPAR) family (2). There are two isoforms of Lipin-1 (alpha and beta) which appear to serve distinct functions in adipocytes, with unique expression dynamics, subcellular localization, and effects on gene expression. Lipin-1B is the predominant form in mature adipocytes. Lipin-1 expression levels are associated with insulin sensitivity, metabolic rate, hypertension, and risk for the metabolic syndrome (4). |
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Western blot - Lipin 1 antibody [EPR3725]; All lanes : Anti-Lipin 1 antibody [EPR3725] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : HuT-78 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 99 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lipin 1 antibody [EPR3725]; ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human colon tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lipin 1 antibody [EPR3725]; ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human uterus tissue.
Immunocytochemistry/ Immunofluorescence - Lipin 1 antibody [EPR3725]; Immunofluorescent staining of HepG2 cells using ab92316 at 1/100 dilution.
Flow Cytometry-Anti-Lipin 1 antibody [EPR3725](ab92316); Overlay histogram showing HepG2 cells stained with ab92316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.