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Home Antibody All anti-LIN28A antibodies
UltraMAB

Anti-LIN28A GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590856 Rabbit Polyclonal Lin28 Antibody 100ug $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)
ELISA(1)
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Also for LIN28A (NM_024674)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.12 mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens lin-28 homolog A (C. elegans) (LIN28A)
Alternative NameCSDD1; LIN-28; lin-28A; LIN28; ZCCHC1
Database LinkNP_078950
Entrez Gene 79727 Human
FunctionLIN28 , also known as CSDD1, ZCCHC1, was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN28A (RC203397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN28A.
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ELISA: LIN28 Antibody
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Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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