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Anti-LIN28A Antibody EPR4640

WB(1)

IHC(1)

IF(1)

Gene Name Homo sapiens lin-28 homolog A (C. elegans) (LIN28A) Synonyms: CSDD1; LIN-28; lin-28A; LIN28; ZCCHC1 Immunogen A synthetic peptide corresponding to residues in human LIN28A was used as an immunogen. Buffer Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt. Clone Name EPR4640 Isotype Species Reactivity Human Concentration Purification (Protein A or G Sepharose) Guaranteed Application * WB, IHC, IF Suggested Dilutions WB: 1:10,000 - 50,000; IHC 1:100 - 250; ICC: 1:25 - 50; Background LIN28A, a promoter of pluripotency, is a developmentally regulated RNA binding protein, which selectively blocks the processing of pri-let-7 miRNAs in embryonic cells (1,2). One of the let-7 family members, let-7g targets and represses LIN28 through four 3'-UTR binding sites. It has been reported that LIN28/let-7 pair has the potential to act as a 'toggle switch' that balances the decision to maintain pluripotency vs. differentiation (3). LIN28 was identified as a negative regulator of miRNA biogenesis and suggested that it may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (1). Related Pathway

Related Lysate from OriGene • Transient overexpression lysate of lin-28 homolog (C. elegans) (LIN28) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • LIN28A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • LIN28A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Lin28 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • LIN28A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Lin28a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens lin-28 homolog A (C. elegans) (LIN28A) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens lin-28 homolog A (C. elegans) (LIN28A) as transfection-ready DNA • Lenti ORF clone of Human lin-28 homolog A (C. elegans) (LIN28A) , Myc-DDK-tagged • Lenti ORF clone of Human lin-28 homolog A (C. elegans) (LIN28A), mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens lin-28 homolog A (C. elegans) (LIN28A) as 200 ug transfection-ready DNA Related cDNA Clones • Homo sapiens lin-28 homolog A (C. elegans) (LIN28A) as transfection-ready DNA

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

• HRP
• Biotin
• PE
• FITC
• DyLight® 488
• DyLight® 550
• DyLight® 594
• DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)