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Anti-LIMS1 Antibody EP1943Y
Also for LIMS1 (NM_004987)
|A synthetic peptide corresponding to residues within LIM zinc-binding 3 region of human LIMS1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2|
|PINCH; PINCH-1; PINCH1|
|The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. [provided by RefSeq]. |
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Western blot - LIMS1 antibody [EP1943Y]; All lanes : Anti-LIMS1 antibody [EP1943Y] at 1/5000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : K562 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : 293 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 34-37 kDa .