LGR5 (leucine-rich repeat containing G protein-coupled receptor 5) An Orphan GPCR protein, contains large extracellular domain, Stem cell biomarker for small intestine, active in colon cancer; overexpressed in ovary and liver tumors.
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HT29 cells are detached, fixed with 2% formaldehyde for 10 min, and permeabilized with 0.1% trixon-X100. After blocking, the cells are stained with Dylight633-conjugated mouse IgG1 (red line) or Dylight633-conjugated LGR5 antibodies (blue line).
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