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Home All anti-LEF1 antibodies
Anti-LEF1 Antibody EP2030Y
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for LEF1 (NM_016269)
OriGene Data
| A synthetic peptide corresponding to residues in human LEF-1/TCF-1 alpha was used as an immunogen. |
| EP2030Y |
Rabbit IgG |
| Human |
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| WB, IHC |
FC: 1:20, ICC: 1:100 - 250, IHC: 1:50, WB: 1:1,000 - 2,000, |
| 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1 |
| LEF-1; TCF10; TCF1ALPHA; TCF7L3 |
| NP_057353 |
| The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein, allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3). |
Adherens junction Delta-Notch Signaling Pathway Melanogenesis TGF Beta Signaling Pathway Wnt Signaling Pathway |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
A. Western blot analysis on Jurkat cell lysate using anti- LEF-1/TCF-1 alpha RabMAb .
IHC Image
B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-LEF-1/TCF-1 alpha RabMAb .
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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