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Anti-LDLR Antibody EP1553Y
Also for LDLR (NM_000527)
|A synthetic peptide corresponding to residues on the C-terminus of human LDL receptor was used as an immunogen. This antibody detects both isoforms of LDL receptor.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:5000; IP: 1:50; FC: 1:70 - 1:100; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens low density lipoprotein receptor (LDLR), transcript variant 1|
|FH; FHC; LDLCQ2|
|The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis (1). Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits (2). It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) was shown to be mediated by LDL receptors on cultured cells (3). |
Wnt Signaling Pathway
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Western blot - LDL Receptor antibody [EP1553Y]; Anti-LDL Receptor antibody [EP1553Y] at 1/500 dilution + Human Plasma Total Protein Lysate at 10 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 95 kDa.Observed band size : 100 kDa .Additional bands at : 27 kDa,48 kDa. We are unsure as to the identity of these extra bands.Exposure time : 4 minutes
Immunohistochemistry (Paraffin-embedded sections) - LDL Receptor antibody [EP1553Y]; ab52818 at 1/100 dilution staining LDL receptor in human liver by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - LDL Receptor antibody [EP1553Y]; ab52818 at 1/100 dilution staining LDL receptor in HepG2 cells by Immunocytochemistry.
Immunocytochemistry/ Immunofluorescence - LDL Receptor antibody [EP1553Y]; ICC/IF image of ab52818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-LDL Receptor antibody [EP1553Y](ab52818); Overlay histogram showing U937 cells stained with ab52818 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.