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Anti-LCP2 PHOSPHO Antibody EP2853Y
Also for LCP2 (NM_005565)
|A phospho specific peptide corresponding to residues surrounding tyrosine 145 of human SLP-76. This antibody detects SLP-76 phosporylated on tyr145.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:100; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) (LCP2)|
|The activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kDa protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that SLP76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of SLP76 cDNA gives rise to a single product of 76 kDa that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. It has been reported that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that SDF-1alpha stimulation of prolonged ERK activation in Jurkat T cells requires both the ZAP-70 tyrosine kinase and the Src homology 2 domain-containing leukocyte protein of SLP-76 scaffold protein. This pathway involves ZAP-70-dependent tyrosine phosphorylation of SLP-76 at one or more of its tyrosines, 113, 128, and 145 (2). |
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Western blot - SLP76 (phospho Y145) antibody [EP2853Y]; All lanes : Anti-SLP76 (phospho Y145) antibody [EP2853Y] at 1/200000 dilution.Lane 1 : Jurkat cell lysates un-treated.Lane 2 : Jurkat cell lysates treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 76 kDa .
Flow Cytometry - SLP76 (phospho Y145) antibody [EP2853Y]; Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate treated (red) using ab75829 at a 1/100 dilution. Also, pervanadate Jurkat cells using the same antibody preincubated with phospho SLP76 peptide (blue) or non phospho SLP76 peptide (orange).