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Home Antibody All anti-LCP2 antibodies

Anti-LCP2 PHOSPHO Antibody EP2853Y

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SKU Description Amount Price Availability*  
TA301257 Rabbit Monoclonal Antibody against LCP2 (Clone EP2853Y) (Phospho-specific) 100ul $325 In Stock

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WB(1)
FC(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding tyrosine 145 of human SLP-76. This antibody detects SLP-76 phosporylated on tyr145.
Clone NameEP2853Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000 - 1:10000; ICC: 1:100; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) (LCP2)
Alternative NameSLP-76; SLP76
Database LinkNP_005556
Entrez Gene 3937 Human
FunctionThe activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kDa protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that SLP76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of SLP76 cDNA gives rise to a single product of 76 kDa that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. It has been reported that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that SDF-1alpha stimulation of prolonged ERK activation in Jurkat T cells requires both the ZAP-70 tyrosine kinase and the Src homology 2 domain-containing leukocyte protein of SLP-76 scaffold protein. This pathway involves ZAP-70-dependent tyrosine phosphorylation of SLP-76 at one or more of its tyrosines, 113, 128, and 145 (2).
Related Pathway Natural killer cell mediated cytotoxicityT cell receptor signaling pathwayFc epsilon RI signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - SLP76 (phospho Y145) antibody [EP2853Y]; All lanes : Anti-SLP76 (phospho Y145) antibody [EP2853Y] at 1/200000 dilution.Lane 1 : Jurkat cell lysates un-treated.Lane 2 : Jurkat cell lysates treated with pervanadate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.Observed band size : 76 kDa .
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Flow Cytometry - SLP76 (phospho Y145) antibody [EP2853Y]; Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate treated (red) using TA301257 at a 1/100 dilution. Also, pervanadate Jurkat cells using the same antibody preincubated with phospho SLP76 peptide (blue) or non phospho SLP76 peptide (orange).
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