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Home Antibody All anti-KRT8 antibodies

Anti-KRT8 TRUEMAB Antibody clone 2E4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500022
  • CK8 (Keratin 8) mouse monoclonal antibody, clone 2E4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419425) , 20ug
100ul 325 In Stock
CF500022
  • Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone 2E4
100ug $450 3-4 weeks
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WB(2)
IHC(1)
IF(2)
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Also for KRT8 (NM_002273)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Clone Nameclone 2E4 IsotypeIgG1
Species ReactivityHuman Concentration1.46mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~1000, IHC 1:50, IF 1:50~100,
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens keratin 8 (KRT8), transcript variant 2
Alternative NameCARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Database LinkNP_002264
FunctionKeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Related Pathway
EGFR1 Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500022)
IF Image
Anti-KRT8 mouse monoclonal antibody (TA500022) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
IF Image
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500022).

 

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