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Anti-KRT19 Antibody EPR1579Y
Also for KRT19 (NM_002276)
|A synthetic peptide corresponding to residues near the N-terminus of human CK-19 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1: 10,000~50,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:20; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens keratin 19 (KRT19)|
|CK19; K19; K1CS|
|Cytokeratin polypeptide 19 (CK19) is a type I intermediate filament protein that is expressed in stratified and simple-type epithelia (1). Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex, perhaps through the interaction of the cytokeratins with the actin-binding domain of dystrophin. Studies in COS-7 cells and in vitro showed that dystrophin binds directly and specifically to K19. Results show that dystrophin interacts through its actin-binding domain with CK19 specifically and are consistent with the idea that cytokeratins associate with dystrophin at the sarcolemma of striated muscle (2). CK19 is synthesized mainly in embryonic and adult simple epithelia, but has also been found in stratified epithelia as well. CK19 is the smallest known keratin and is remarkable in that, contrary to all other keratins, it does not have a designated partner for the formation of filaments, implying that regulation of its expression is different from other keratin-encoding genes (3). |
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Western blot - Cytokeratin 19 antibody [EPR1579Y]; All lanes : Anti-Cytokeratin 19 antibody [EPR1579Y] at 1/10000 dilution.Lane 1 : T47D cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : SKBR-3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 44 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 19 antibody [EPR1579Y]; Immunohistochemical staining of human stomach adenocarcinoma tissue with 1/100-1/250 ab76539.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 19 antibody [EPR1579Y](ab76539); Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab76539. Green- CK19 red-PI.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EPR1579Y]; All lanes : Anti-Cytokeratin 19 antibody [EPR1579Y] at 1/10000 dilution.Lane 1 : T47D cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : SKBR-3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 44 kDa.
Flow Cytometry - Anti-Cytokeratin 19 antibody [EPR1579Y]; Overlay histogram showing MCF7 cells stained with ab76539 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.