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Home Antibody All anti-KRT18 antibodies

Anti-KRT18 PHOSPHO Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA321085 Anti-KRT18 (Phospho-Ser33) Rabbit Polyclonal Antibody 100ul $325 2-3 Weeks
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WB(1)
IHC(1)
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Also for KRT18 (NM_000224)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from Human Keratin 18 (CK18).
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
Predicted MW Explanation 48kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens keratin 18, type I (KRT18), transcript variant 1
Alternative NameCYK18; K18
Database LinkNP_000215
Entrez Gene 3875 Human
FunctionKRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Related Pathway Pathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Predicted band size: 48 kDa . Positive control: HT29 cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HT29 cells untreated with EGF lysate Lane2: HT29 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
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Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using KRT18 (Phospho-Ser33) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
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