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Anti-KRT16 Antibody EP1615Y
Also for KRT16 (NM_005557)
|A synthetic peptide corresponding to residues near the N-terminus of human CK-16 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||IHC-P: 1:100 - 1:250; WB: 1:5000 - 1:10000; IP: 1:30; ICC: 1:100 - 1:250; FC: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens keratin 16 (KRT16)|
|CK16; FNEPPK; K16; K1CP; KRT16A; NEPPK|
|The human type I keratin 16 (CK16) is constitutively expressed in a number of complex epithelial tissues, including skin, but is better known for its induction under conditions favoring enhanced proliferation or abnormal differentiation, including wound healing, psoriasis, and cancer (1). CK16 is expressed in suprabasal interfollicular epidermis in wound healing and other pathological conditions associated with hyperproliferation, such as psoriasis and are induced when keratinocytes are cultured in vitro. However, CK16 is also constitutively expressed in normal suprabasal mucosal and palmoplantar keratinocytes (2). Pachyonychia congenita (PC) is a group of inherited ectodermal dysplasias, the characteristic phenotype being hypertrophic nail dystrophy. The PC-1 phenotype may be distinguished by the absence of the epidermal cysts found in PC-2, and it has been shown to be caused by mutations in either keratin K16 or its expression partner, the K6a isoform of K6. Mutations in K16 have also been shown to cause a milder related phenotype, focal non-epidermolytic palmoplantar keratoderma (3). |
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Western blot - Cytokeratin 16 antibody [EP1615Y]; Anti-Cytokeratin 16 [EP1615Y] antibody at 1/10000 dilution + HaCat cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 16 antibody [EP1615Y]; Immunohistochemical analysis of Cytokeratin 16 in paraffin embedded human squamous cervical carcinoma tissue using ab76416 at a 1/100 dilution.
Flow Cytometry - Cytokeratin 16 antibody [EP1615Y]; Overlay histogram showing HepG2 cells stained with ab76416 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.