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Anti-KITLG Antibody EP665Y
Also for KITLG (NM_000899)
|A synthetic peptide corresponding to residues near the N-terminus of human SCF was used as an immungen.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:10000; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens KIT ligand (KITLG), transcript variant b|
|FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7|
Entrez Gene 4254 Human
|Novel hematopoietic growth factor, stem cell factor (SCF), was cloned and demonstrated to be the ligand for the c-kit tyrosine kinase receptor. SCF is encoded by Sl (steel), a gene critical to the development of several distinct cell lineages during embryonic life and which has important effects on hematopoiesis in the adult (1). SCF is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF exhibits potent synergistic activities in conjunction with colony-stimulating factors, resulting in increased colony numbers and colony size (2). |
|TransmembraneDruggable Genome Cytokine-cytokine receptor interactionHematopoietic cell lineageMelanogenesisPathways in cancer|
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Western blot - SCF antibody [EP665Y]; Anti-SCF [EP665Y] antibody at 1/10000 dilution + recombinant protein (5ng).Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 18 kDa .
Immunohistochemistry (Paraffin-embedded sections) - SCF antibody [EP665Y]; Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using TA300957 at a dilution of 1/100-1/250.
Flow Cytometry - SCF antibody; Overlay histogram showing MCF-7 cells stained with TA300957 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in MCF-7 cells under the same conditions.