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Home Antibody All anti-KITLG antibodies

Anti-KITLG Antibody EP665Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300957
  • Rabbit Monoclonal Antibody against KITLG (Clone EP665Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400322) , 20ug Explanation
100uL 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for KITLG (NM_000899)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human SCF was used as an immungen.
Clone NameEP665Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 31 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens KIT ligand (KITLG), transcript variant b
Alternative NameFPH2; Kitl; KL-1; MGF; SCF; SF; SHEP7
Database LinkNP_000890
Entrez Gene 4254 Human
FunctionNovel hematopoietic growth factor, stem cell factor (SCF), was cloned and demonstrated to be the ligand for the c-kit tyrosine kinase receptor. SCF is encoded by Sl (steel), a gene critical to the development of several distinct cell lineages during embryonic life and which has important effects on hematopoiesis in the adult (1). SCF is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF exhibits potent synergistic activities in conjunction with colony-stimulating factors, resulting in increased colony numbers and colony size (2).
Related PathwayTransmembraneDruggable Genome Cytokine-cytokine receptor interactionHematopoietic cell lineageMelanogenesisPathways in cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SCF antibody [EP665Y]; Anti-SCF [EP665Y] antibody at 1/10000 dilution + recombinant protein (5ng).Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 18 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - SCF antibody [EP665Y]; Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using TA300957 at a dilution of 1/100-1/250.
FC Image
Flow Cytometry - SCF antibody [EP665Y]; Overlay histogram showing MCF-7 cells stained with TA300957 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

 

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