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Home Antibody All anti-KIT antibodies

Anti-KIT PHOSPHO Antibody

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SKU Description Amount Price Availability*  
TA323712 Anti-KIT (phospho-Tyr936) Rabbit Polyclonal Antibody 100ul $325 2-3 Weeks
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WB(1)
IHC(1)

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 936 (H-I-Y(p)-S-N) derived from Human c-Kit.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 1
Alternative NameC-Kit; CD117; PBT; SCFR
Database LinkNP_000213
Entrez Gene 3815 Human
FunctionThis is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)
Related PathwayProtein KinaseES Cell Differentiation/IPSTransmembraneStem cell - PluripotencyAdult stem cellsEmbryonic stem cellsDruggable Genome Cytokine-cytokine receptor interactionEndocytosisHematopoietic cell lineageMelanogenesisPathways in cancerAcute myeloid leukemiaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Predicted band size: 120; 145 kDa . Positive control: Hela cell treated with SC lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Hela cell untreated with SCF lysate Lane2: Hela cell treated with SCF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
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Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using KIT (phospho-Tyr936) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
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