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Anti-KEAP1 TRUEMAB Antibody Clone OTI1B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502059 KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4) 100ul
30ul
$379 In Stock
LC404250 KEAP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502059 Carrier-free (BSA/glycerol-free) KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(3)
IHC(3)
FC(3)
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Also for KEAP1 (NM_203500)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.
Clone NameClone OTI1B4 IsotypeIgG1
Species ReactivityHuman, Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 69.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1
Alternative NameINrf2; KLHL19
Database LinkNP_987096
Entrez Gene 9817 Human
Entrez Gene 50868 Mouse
FunctionThis gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq].
Related PathwayTranscription Factors Ubiquitin mediated proteolysis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Figure from citation: Western Blot of Keap1 protein level by using anti-Keap1 antibody in human BEAS2B cells. View Citation
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502059)
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HEK293T cells transfected with either RC202189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KEAP1 antibody(TA502059), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).
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