Anti-KEAP1 Antibody, Clone 1B4

Also for KEAP1 (NM_203500)

OriGene Data

Immunogen	Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.
Clone Name	Clone 1B4	Isotype	IgG1
Species Reactivity	Human, Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1
Alternative Name	INrf2; KLHL19
Database Link	NP_987096
Function	This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody. (TA502059)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody. (TA502059)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody. (TA502059)

FC Image

HEK293T cells transfected with either RC202189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KEAP1 antibody(TA502059), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-KEAP1 antibody(TA502059),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-KEAP1 TrueMAB Antibody Clone 1B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data