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Home Antibody All anti-JUB antibodies

Anti-JUB Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319508 Rabbit polyclonal anti-Ajuba antibody 100ug $325 3-7 Days
LC409908 AJUBA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.67 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:20,000 - 1:80,000, WB: 1:500 - 1:2,500, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Human Ajuba (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A.  Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Reference Data

Target NameHomo sapiens ajuba LIM protein (AJUBA), transcript variant 1
Alternative NameJUB
Database LinkNP_116265
Entrez Gene 84962 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB using Anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head).  Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 µg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.  Detection occurred using a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT.
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WB using Anti-Ajuba antibody shows detection of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead).  Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates.IP of Ajuba followed by WB may result in cleaner background staining.Primary antibody was used at 1:500.  Detection occurred using a 1:5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG.
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