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Anti-Ido1 Antibody 10.1
Also for Ido1 (NM_002164)
|Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.|
||ELISA: 1:5000-1:50000, WB: 1:500-1:1500, IHC: 1:100-1:500, IF: 1:50-1:100, IP: 10-100 uL
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
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Western Blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells. Lane 2: IFN-r treated HeLa cells. Load: 35 µg per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.