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Home Antibody All anti-ITK antibodies

Anti-ITK Antibody Y401


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300548 Rabbit Monoclonal Antibody against ITK (Clone Y401) 100ul $325 In Stock
LC401706 ITK HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human Emtk/Itk/Tsk was used as immunogen.
Clone NameY401 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens IL2-inducible T-cell kinase (ITK)
Alternative NameEMT; LPFS1; LYK; PSCTK2
Database LinkNP_005537
Entrez Gene 3702 Human
FunctionEmtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).
Related PathwayProtein KinaseDruggable Genome Chemokine signaling pathwayT cell receptor signaling pathwayLeukocyte transendothelial migration

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ITK antibody [Y401]; Anti-ITK antibody [Y401] at 1/5000 dilution + Jurkat cell lysate.Predicted band size : 72 kDa.Observed band size : 68 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - ITK antibody [Y401]; Ab32039 at a 1/250 dilution, staining ITK in paraffin embedded human lymph node tissue by Immunohistochemistry.

FC Image
Flow Cytometry-Anti-ITK antibody(TA300548); Overlay histogram showing Jurkat cells stained with TA300548 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (1ug/1x10^6 cells ) used under the same conditions. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


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