OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-ITK antibodies

Anti-ITK Antibody Y401

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300548
  • Rabbit Monoclonal Antibody against ITK (Clone Y401)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401706) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for ITK (NM_005546)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human Emtk/Itk/Tsk was used as immunogen.
Clone NameY401 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; FC: 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens IL2-inducible T-cell kinase (ITK)
Alternative NameEMT; LPFS1; LYK; PSCTK2
Database LinkNP_005537
FunctionEmtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ITK antibody [Y401]; Anti-ITK antibody [Y401] at 1/5000 dilution + Jurkat cell lysate.Predicted band size : 72 kDa.Observed band size : 68 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - ITK antibody [Y401]; Ab32039 at a 1/250 dilution, staining ITK in paraffin embedded human lymph node tissue by Immunohistochemistry.
FC Image
Flow Cytometry-Anti-ITK antibody [Y401](TA300548); Overlay histogram showing Jurkat cells stained with TA300548 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title