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Anti-ITK Antibody Y402


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against ITK (Clone Y402)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401706) , 20ug Explanation
100ul $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human Emtk/Itk/Tsk was used as immunogen.
Clone NameY402 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:1000; FC: 1:50 - 1:1000; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC/IF or IHC.

Reference Data

Target NameHomo sapiens IL2-inducible T-cell kinase (ITK)
Alternative NameEMT; LPFS1; LYK; PSCTK2
Database LinkNP_005537
Entrez Gene 3702 Human
FunctionEmtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).
Related PathwayProtein KinaseDruggable Genome Chemokine signaling pathwayT cell receptor signaling pathwayLeukocyte transendothelial migration

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - ITK antibody [Y402]; Anti-ITK antibody [Y402] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Flow Cytometry - Anti-ITK antibody [Y402]; Overlay histogram showing Jurkat cells stained with TA300522 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


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